It is possible that other miRNAs, including those in the let-7 family, control developmental timing in other tissues during the recovery phase after L1 starvation. Although the complete removal of miRNA functions causes embryonic lethality or infertility in worms, a partial disruption of overall miRNA functions by mutating either ain-1 or ain-2 provides an effective way to investigate miRNA functions (16, 17). However, we found that the reporter transgene with the lin-42 3′UTR was significantly repressed in wild-type worms, but derepressed in the mir-71(lf) worms (Fig. 4 H and I).
Tap Scan QR code and scan the QR code from your third-party account 2FA setup screen, or, to recover a Duo-protected account, access the My Settings and Devices page from the Duo prompt to reactivate the account. See third-party account recovery on Android in action. To use Instant Restore you must have previously backed up your Duo Mobile accounts to Google Drive. If you created a Google Drive backup using the old Duo Restore toggle and know the recovery password you set when enabling Duo Mobile backup, you can still access this backup to perform an Instant Restore. If you have Google account backup disabled or your device does not have a PIN, pattern, or password screen lock, please see Enabling Duo Restore for the legacy Instant Restore flow. You’ll still need to perform the Instant Restore steps before you can use those accounts to log in to Duo-protected services with Duo Push or Duo Mobile passcodes.
(D) Bar graph showing that the delayed VPC timing defect of mir-71(lf) worms was enhanced by daf-16(lf) after 1 or 3 d of L1 starvation. (B) Bar graph showing the correlation between the severity of the retarded vulval precursor cell (VPC) timing defect of mir-71(lf) mutants and the duration of L1 starvation. Whereas the vulva of wild-type worms developed into the pyramidal stage (81 of 82 worms), the P6.p of mir-71(n4115, lf) mutant worms divided only once (83 of 89 worms). The computation-based prediction that age-1 and pdk-1 are potential targets of miR-71 was also reported in a recent study focusing on miRNA functions in aging where the mRNA level of pdk-1 was shown to be up-regulated in mir-71 worms (14). (C) Fluorescence and differential interference contrast (DIC) images showing that the age-1 3′UTR reporter was repressed in mir-71(+) worms (3/4 transgenic lines) but not in mir-71(lf) worms (4/4 transgenic lines).
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If you become locked out of those services and don’t have a backup of your accounts in Duo Mobile, you’ll need to contact the support team for that application (or perform the account recovery process for each of those third-party applications). However, whether an account can be restored depends upon Duo Restore being enabled by the administrator in the Duo Admin Panel or whether you’ve set a recovery password for reconnecting third-party accounts. Individual GFP reporter constructs for candidate genes (4 ng/μL) and the mCherry internal control plasmid (4 ng/μL) were mixed with unc-119 rescuing plasmid (20 ng/μL) and pBluescript KS+ (72 ng/μL) and coinjected into unc-119(ed3) and mir-71(n4115); unc-119(ed3) worms following standard protocols (32). Knocking down lit-1 by RNAi in mir-71(lf); lin-42(lf) double mutants caused no significant suppression of the VPC timing defects of mir-71(lf) worms. To determine the functional relationship of miR-71 with LIN-42 and LIT-1, mir-71(lf); lin-42(lf) L1 worms were starved for 4 d and recovered on lit-1(RNAi) plates. We then compared the expression of a hbl-1 3′UTR reporter (18) in the mir-71(lf) mutants with that in wild type and found that the expression of this reporter was slightly derepressed at L3 in the mir-71 mutant (Fig. 4 F and G).
Remember, Duo Support can’t recover your third-party accounts for you or reset your third-party recovery password. If you didn’t enable backups for your third-party accounts when you added the first one, you can do it at any time. You’ll need to provide it again to recover these accounts.
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{Note that this doesn’t reconnect your Duo-protected accounts. This process doesn’t reconnect any third-party accounts. To use Instant Restore you must have previously backed up your device with iCloud (with iCloud Keychain on) or an encrypted iTunes or Finder backup. If you lose this password you’ll need to manually reconnect your third-party accounts by visiting each of those services individually and following their 2FA setup process. When Duo Mobile detects you have a third-party account, you’ll be prompted to create a recovery password.}
